Modeling K,ATP-Dependent Excitability in Pancreatic Islets
نویسندگان
چکیده
منابع مشابه
Modeling K,ATP--dependent excitability in pancreatic islets.
In pancreatic ?-cells, K,ATP channels respond to changes in glucose to regulate cell excitability and insulin release. Confirming a high sensitivity of electrical activity to K,ATP activity, mutations that cause gain of K,ATP function cause neonatal diabetes. Our aim was to quantitatively assess the contribution of K,ATP current to the regulation of glucose-dependent bursting by reproducing exp...
متن کاملAbnormalities of pancreatic islets by targeted expression of a dominant-negative KATP channel.
ATP-sensitive K+ (KATP) channels are known to play important roles in various cellular functions, but the direct consequences of disruption of KATP channel function are largely unknown. We have generated transgenic mice expressing a dominant-negative form of the KATP channel subunit Kir6.2 (Kir6.2G132S, substitution of glycine with serine at position 132) in pancreatic beta cells. Kir6.2G132S t...
متن کاملEXPERIMENTAL STUDY Glucose triggers protein kinase A-dependent insulin secretion in mouse pancreatic islets through activation of the KATP channel-dependent pathway
Objective: To assess the significance of protein kinase A (PKA) in glucose triggering of ATP-sensitive Kþ (KATP) channel-dependent insulin secretion and in glucose amplification of K þ ATP channel-independent insulin secretion. Methods: Insulin release from cultured perifused mouse pancreatic islets was determined by radioimmunoassay. Results: In islets cultured at 5.5 mmol/l glucose, and then ...
متن کاملCalcium-calmodulin-dependent myosin phosphorylation by pancreatic islets.
Pancreatic islets contain enzyme activity which catalyzes the phosphorylation by MgATP of cardiac, skeletal, or smooth muscle myosin light chains. The enzyme is activated by calcium (Ka = 10 microM) and calmodulin (Ka = 2 nM) and inhibited by trifluoperazine (Ki = 10 microM), a known inhibitor of calmodulin and of insulin secretion. The enzyme binds to a calmodulin affinity column when Ca2+ is ...
متن کاملProtein phosphatases in pancreatic islets.
The prevalence of diabetes is increasing rapidly worldwide. A cardinal feature of most forms of diabetes is the lack of insulin-producing capability, due to the loss of insulin-producing β-cells, impaired glucose-sensitive insulin secretion from the β-cell, or a combination thereof, the reasons for which largely remain elusive. Reversible phosphorylation is an important and versatile mechanism ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2014
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2014.09.037